Coding

Part:BBa_K5218001:Design

Designed by: Rui Zhang, Lichuan Chen, Xiaojuan Wang   Group: iGEM24_BGI-MammothEdu-South   (2024-08-10)


RcTAL


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 490
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 490
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 490
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 490
    Illegal AgeI site found at 646
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

The CDS sequence was obtained from published article[1] and De novo synthesised.

References

[1] Kyndt J A , Meyer T E , Cusanovich M A ,et al.Characterization of a bacterial tyrosine ammonia lyase, a biosynthetic enzyme for the photoactive yellow protein[J].Febs Letters, 2002, 512(1-3):240-244.DOI:10.1016/S0014-5793(02)02272-X.